anti gal4 ad sc 1663 antibodies (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
anti gal4 ad sc 1663 antibodies
![SUP physically interacts with TPL through its EAR motif (DLELRL). ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the <t>GAL4</t> DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLE A R A ) or mSUP (DLE G R G ) [each fused to the <t>GAL4</t> <t>activation</t> <t>domain</t> (AD)]. Growth on selection (right panel) detects a physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) that is weakened by mutation of the EAR motif in SUP (DLE A R A ). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S3](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5690/pmc12595690/pmc12595690__12870_2025_7553_Fig2_HTML.jpg)
Anti Gal4 Ad Sc 1663 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1663/GAL4/pmc12595690-66-28-32
Average 95 stars, based on 744 article reviews
Anti Gal4 Ad Sc 1663 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1663/GAL4/pmc12595690-66-28-32
Average 95 stars, based on 744 article reviews
anti gal4 ad sc 1663 antibodies - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning"
Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning
Journal: BMC Plant Biology
doi: 10.1186/s12870-025-07553-z
Figure Legend Snippet: SUP physically interacts with TPL through its EAR motif (DLELRL). ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLE A R A ) or mSUP (DLE G R G ) [each fused to the GAL4 activation domain (AD)]. Growth on selection (right panel) detects a physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) that is weakened by mutation of the EAR motif in SUP (DLE A R A ). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S3
Techniques Used: Binding Assay, Activation Assay, Selection, In Vitro, Mutagenesis, Staining, Expressing, Western Blot
![CRISPR-generated EAR mutations disrupt physical interaction between SUP and TPL. ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLELL) or mSUP (∆EAR) [each fused to the GAL4 activation domain (AD)]. Growth on selection (right panel) indicates physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) which is weakened by mutation of the EAR motif in CRISPR allele mSUP (DLELL). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S7](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5690/pmc12595690/pmc12595690__12870_2025_7553_Fig5_HTML.jpg "... assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP ...")
Figure Legend Snippet: CRISPR-generated EAR mutations disrupt physical interaction between SUP and TPL. ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLELL) or mSUP (∆EAR) [each fused to the GAL4 activation domain (AD)]. Growth on selection (right panel) indicates physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) which is weakened by mutation of the EAR motif in CRISPR allele mSUP (DLELL). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S7
Techniques Used: CRISPR, Generated, Binding Assay, Activation Assay, Selection, In Vitro, Mutagenesis, Staining, Expressing, Western Blot
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